Anti-Human TIGIT Recombinant Antibody (SAA0153) (HS739107)

Name: Anti-Human TIGIT Recombinant Antibody (SAA0153)
Brand: abinScience
SKU: HS739107
Price: 191 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.	HS739107
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	SAA0153
Clonality	Monoclonal
Target	VSTM3, TIGIT, V-set and transmembrane domain-containing protein 3, VSIG9, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q495A1
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a ~26 kDa protein. Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. 1. Yu, X. et al. (2009) Nature immunology 10, 48-57. PMID: 19011627 2. Liu, S. et al. (2013) Cell death and differentiation 20, 456-64. PMID: 23154388 3. Li, M. et al. (2014) The Journal of biological chemistry 289, 17647-57. PMID: 24817116 4. Reches, A. et al. (2020) Journal for immunotherapy of cancer 8. PMID: 32503945
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human TIGIT antibody.TIGIT Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TIGIT antibody monoclonal antibody (Catalog # HS739107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet