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Anti-Human UCP3 Polyclonal Antibody (HX158014)

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Anti-Human UCP3 Polyclonal Antibody
Anti-Human UCP3 Polyclonal Antibody
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Vista general
Número de catálogoHX158014
Descripción
Anti-Human UCP3 Polyclonal Antibody (HX158014) is a rabbit polyclonal antibody detecting UCP3 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Sus scrofa, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human UCP3 (Met1-Phe312).
Objetivo UCP3
Purificación Purified by antigen affinity column.
Número de acceso P55916
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosMitochondrial uncoupling protein 3, UCP 3, Solute carrier family 25 member 9, UCP3, SLC25A9
Fondo

Putative mitochondrial transporter UCP3 is a ~34 kDa protein. Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism. Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis. However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity.

1. De Marchi, U. et al. (2011) The Journal of biological chemistry 286, 32533-41. PMID: 21775425
2. Codella, R. et al. (2023) FEBS letters 597, 309-319. PMID: 36114012
3. Echtay, KS. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1416-21. PMID: 11171965
4. Zackova, M. et al. (2003) The Journal of biological chemistry 278, 20761-9. PMID: 12670931
5. Jaburek, M. et al. (2003) The Journal of biological chemistry 278, 25825-31. PMID: 12734183
6. Gong, DW. et al. (1997) The Journal of biological chemistry 272, 24129-32. PMID: 9305858
7. Cadenas, S. et al. (2002) The Journal of biological chemistry 277, 2773-8. PMID: 11707458
Nota For research use only.
Imágenes
  • Anti-Human UCP3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-UCP3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate
    Lane 2: Mouse heart lysate
    Lane 3: Rat liver lysate
    Lane 4: Rat heart lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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