Human UCP3 Recombinant Protein (N-His) (HX158012)

Name: Human UCP3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX158012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX158012
Description	Recombinant Human UCP3 Protein, N-His (HX158012) expressed in E. coli, spanning Met1-Phe312. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P55916
Protein length	Met1-Phe312
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.38 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	UCP3, Solute carrier family 25 member 9, UCP 3, SLC25A9, Mitochondrial uncoupling protein 3
Background	Putative mitochondrial transporter UCP3 is a ~34 kDa protein. Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism. Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis. However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity. 1. De Marchi, U. et al. (2011) The Journal of biological chemistry 286, 32533-41. PMID: 21775425 2. Codella, R. et al. (2023) FEBS letters 597, 309-319. PMID: 36114012 3. Echtay, KS. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1416-21. PMID: 11171965 4. Zackova, M. et al. (2003) The Journal of biological chemistry 278, 20761-9. PMID: 12670931 5. Jaburek, M. et al. (2003) The Journal of biological chemistry 278, 25825-31. PMID: 12734183 6. Gong, DW. et al. (1997) The Journal of biological chemistry 272, 24129-32. PMID: 9305858 7. Cadenas, S. et al. (2002) The Journal of biological chemistry 277, 2773-8. PMID: 11707458
Note	For research use only.

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SDS-PAGE for Recombinant Human UCP3 Protein, N-His

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Datasheet

Human UCP3 Recombinant Protein (N-His).pdf

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Human UCP3 Recombinant Protein (N-His) (HX158012)

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