Anti-Human Vimentin Recombinant Antibody (VB6), FITC (HY550117)

Name: Anti-Human Vimentin Recombinant Antibody (VB6), FITC
Brand: abinScience
SKU: HY550117
Price: 223 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HY550117
Reactivité des espèces	Human
Applications	ELISA, FCM, WB
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	VB6
Conjugaison	FITC
Applications testées	FCM
Cible	Vimentin, VIM, RHC35301
Niveau d'endotoxines	Please contact with the lab for this information.
Produits associés	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Numéro d'accès	P08670
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	Vimentin is a ~53 kDa protein. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using FITC anti-human VIM antibody. U251 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VIM monoclonal antibody (Catalog: HY550117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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