Anti-Human Vimentin Recombinant Antibody (VB6), FITC (HY550117)

Name: Anti-Human Vimentin Recombinant Antibody (VB6), FITC
Brand: abinScience
SKU: HY550117
Price: 223 USD
Availability: InStock

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概要
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データシート
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概要

カタログ番号	HY550117
種反応性	Human
アプリケーション	ELISA, FCM, WB
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1, kappa
クローンID	VB6
抱合	FITC
テスト済みアプリケーション	FCM
ターゲット	Vimentin, VIM, RHC35301
エンドトキシンレベル	Please contact with the lab for this information.
関連製品	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
アクセッション番号	P08670
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	Vimentin is a ~53 kDa protein. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using FITC anti-human VIM antibody. U251 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VIM monoclonal antibody (Catalog: HY550117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献