Anti-Human Vimentin Recombinant Antibody (VB6), FITC (HY550117)

Name: Anti-Human Vimentin Recombinant Antibody (VB6), FITC
Brand: abinScience
SKU: HY550117
Price: 223 USD
Availability: InStock

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Каталожный номер	HY550117
Реактивность видов	Human
Применения	ELISA, FCM, WB
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG1, kappa
ID клона	VB6
Конъюгация	FITC
Проверенные приложения	FCM
Цель	Vimentin, VIM, RHC35301
Уровень эндотоксинов	Please contact with the lab for this information.
Связанные продукты	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Номер доступа	P08670
Форма	Liquid
Буфер хранения	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон	Vimentin is a ~53 kDa protein. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.
Примечание	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Изображения

Flow CytoMetry

Flow-cytometry using FITC anti-human VIM antibody. U251 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VIM monoclonal antibody (Catalog: HY550117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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