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Anti-Human VPS18 Polyclonal Antibody (HD666014)

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Aperçu
Numéro de catalogueHD666014
Description
Anti-Human VPS18 Polyclonal Antibody (HD666014) is a rabbit polyclonal antibody detecting VPS18. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human VPS18 (Glu40-Glu394).
Cible KIAA1475, VPS18, Vacuolar protein sorting-associated protein 18 homolog, hVPS18
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q9P253
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Vacuolar protein sorting-associated protein 18 homolog (VPS18) is a ~110 kDa protein. Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes.

1. Kim, BY. et al. (2001) The Journal of biological chemistry 276, 29393-402. PMID: 11382755
2. Solinger, JA. et al. (2013) The FEBS journal 280, 2743-57. PMID: 23351085
3. Jiang, P. et al. (2014) Molecular biology of the cell 25, 1327-37. PMID: 24554770
4. Wartosch, L. et al. (2015) Traffic (Copenhagen, Denmark) 16, 727-42. PMID: 25783203
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human VPS18 Polyclonal Antibody

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