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Обзор
Каталожный номерHD666014
Описание
Anti-Human VPS18 Polyclonal Antibody (HD666014) is a rabbit polyclonal antibody detecting VPS18. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human VPS18 (Glu40-Glu394).
Цель KIAA1475, VPS18, Vacuolar protein sorting-associated protein 18 homolog, hVPS18
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q9P253
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Фон

Vacuolar protein sorting-associated protein 18 homolog (VPS18) is a ~110 kDa protein. Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes.

1. Kim, BY. et al. (2001) The Journal of biological chemistry 276, 29393-402. PMID: 11382755
2. Solinger, JA. et al. (2013) The FEBS journal 280, 2743-57. PMID: 23351085
3. Jiang, P. et al. (2014) Molecular biology of the cell 25, 1327-37. PMID: 24554770
4. Wartosch, L. et al. (2015) Traffic (Copenhagen, Denmark) 16, 727-42. PMID: 25783203
Примечание For research use only
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Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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