Anti-Human WDR5 IP Recombinant Antibody (SAA0239) (HX013013)

Name: Anti-Human WDR5 IP Recombinant Antibody (SAA0239)
Brand: abinScience
SKU: HX013013
Price: 126 USD
Availability: InStock

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20ug
50ug
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1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HX013013

Reactivité des espèces

Human

Applications

ELISA, FCM, IF, IP

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG1

ID de clone

SAA0239

Cible

WDR5, BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, BIG3

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P61964

RRID

Anti-Human WDR5 IP Antibody (SAA0239) (abinScience Cat# HX013013, RRID:AB_3729773)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

WD repeat-containing protein 5 (WDR5) is a ~36 kDa protein. Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues.

1. Han, Z. et al. (2006) Molecular cell 22, 137-44. PMID: 16600877

2. Couture, JF. et al. (2006) Nature structural & molecular biology 13, 698-703. PMID: 16829960

3. Guelman, S. et al. (2009) Molecular and cellular biology 29, 1176-88. PMID: 19103755

4. Garapaty, S. et al. (2009) The Journal of biological chemistry 284, 7542-52. PMID: 19131338

5. Patel, A. et al. (2009) The Journal of biological chemistry 284, 24242-56. PMID: 19556245

6. Cai, Y. et al. (2010) The Journal of biological chemistry 285, 4268-72. PMID: 20018852

7. Song, JJ. et al. (2008) The Journal of biological chemistry 283, 35258-64. PMID: 18840606

8. Avdic, V. et al. (2011) Structure (London, England : 1993) 19, 101-8. PMID: 21220120

9. Zhang, P. et al. (2012) Nucleic acids research 40, 4237-46. PMID: 22266653

Note

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Images

Flow Cytometry

Flow-cytometry using anti-human WDR5 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human WDR5 antibody monoclonal antibody (Catalog # HX013013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human WDR5 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human WDR5 antibody monoclonal antibody (Catalog # HX013013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human WDR5 IP Antibody (SAA0239).

Reférences

Fiche technique

Anti-Human WDR5 IP Recombinant Antibody (SAA0239).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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