Anti-KISS1 Polyclonal Antibody (HS770014)

Name: Anti-KISS1 Polyclonal Antibody
Brand: abinScience
SKU: HS770014
Price: 168 USD
Availability: InStock

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50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HS770014

Description

Anti-KISS1 Polyclonal Antibody (HS770014) is a rabbit polyclonal antibody detecting KISS1 in ELISA, IHC, WB. Suitable for Human and Macaca mulatta.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human KISS1 (Glu20-Gly138).

Cible

Metastasis-suppressor KiSS-1, Kisspeptin-54, Kisspeptin-1, KISS1

Purification

Purified by antigen affinity column.

Numéro d'accès

Q15726

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Metastasis-suppressor KiSS-1 (KISS1) is a ~14 kDa protein. Kisspeptins are ligands for the G-protein coupled receptor KISS1R/GPR54. The hypothalamic KISS1/KISS1R signaling system plays a central role in the regulation of the hypothalamic-pituitary-gonadal reproductive axis by modulating the secretion of gonadotropin-releasing hormone (GnRH) from GnRH neurons. In these neurons, kisspeptin binding to its receptor activates G(q)-dependent signaling, leading to phospholipase C (PLC) activation, and hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2). The subsequent rise in intracellular calcium levels results in the inhibition of inward rectifier potassium channels and activation of TRPC-like cation channels, leading to GnRH neurons depolarization and stimulation. In addition to this pathway, kisspeptin also triggers G(q)-independent signaling via beta-arrestin, leading to MAPK cascade activation and ERK1/ERK2 phosphorylation.

1. Ohtaki, T. et al. (2001) Nature 411, 613-7. PMID: 11385580

2. Kotani, M. et al. (2001) The Journal of biological chemistry 276, 34631-6. PMID: 11457843

3. Hori, A. et al. (2001) Biochemical and biophysical research communications 286, 958-63. PMID: 11527393

4. Takino, T. et al. (2003) Oncogene 22, 4617-26. PMID: 12879005

5. Bilban, M. et al. (2004) Journal of cell science 117, 1319-28. PMID: 15020672

6. Becker, JA. et al. (2005) Biochemical and biophysical research communications 326, 677-86. PMID: 15596153

7. Matsui, H. et al. (2004) Biochemical and biophysical research communications 320, 383-8. PMID: 15219839

8. Semple, RK. et al. (2005) The Journal of clinical endocrinology and metabolism 90, 1849-55. PMID: 15598687

9. Topaloglu, AK. et al. (2012) The New England journal of medicine 366, 629-35. PMID: 22335740

10. Ahow, M. et al. (2014) Endocrinology 155, 4433-46. PMID: 25147978

Note

For research use only.

Images

Reférences

Fiche technique

Anti-KISS1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-KISS1 Polyclonal Antibody (HS770014)

Aperçu

Images

Reférences

Fiche technique

SDS