ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
14.84 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Metastasis-suppressor KiSS-1 (KISS1) is a ~14 kDa protein. Kisspeptins are ligands for the G-protein coupled receptor KISS1R/GPR54. The hypothalamic KISS1/KISS1R signaling system plays a central role in the regulation of the hypothalamic-pituitary-gonadal reproductive axis by modulating the secretion of gonadotropin-releasing hormone (GnRH) from GnRH neurons. In these neurons, kisspeptin binding to its receptor activates G(q)-dependent signaling, leading to phospholipase C (PLC) activation, and hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2). The subsequent rise in intracellular calcium levels results in the inhibition of inward rectifier potassium channels and activation of TRPC-like cation channels, leading to GnRH neurons depolarization and stimulation. In addition to this pathway, kisspeptin also triggers G(q)-independent signaling via beta-arrestin, leading to MAPK cascade activation and ERK1/ERK2 phosphorylation.
1. Ohtaki, T. et al. (2001) Nature 411, 613-7. PMID: 11385580
2. Kotani, M. et al. (2001) The Journal of biological chemistry 276, 34631-6. PMID: 11457843
3. Hori, A. et al. (2001) Biochemical and biophysical research communications 286, 958-63. PMID: 11527393
4. Takino, T. et al. (2003) Oncogene 22, 4617-26. PMID: 12879005
5. Bilban, M. et al. (2004) Journal of cell science 117, 1319-28. PMID: 15020672
6. Becker, JA. et al. (2005) Biochemical and biophysical research communications 326, 677-86. PMID: 15596153
7. Matsui, H. et al. (2004) Biochemical and biophysical research communications 320, 383-8. PMID: 15219839
8. Semple, RK. et al. (2005) The Journal of clinical endocrinology and metabolism 90, 1849-55. PMID: 15598687
9. Topaloglu, AK. et al. (2012) The New England journal of medicine 366, 629-35. PMID: 22335740
10. Ahow, M. et al. (2014) Endocrinology 155, 4433-46. PMID: 25147978
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human KISS1 Protein, N-His
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