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Anti-Human LPL/LIPD Polyclonal Antibody (HY363014)

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Aperçu
Numéro de catalogueHY363014
Description
Anti-Human LPL/LIPD Polyclonal Antibody (HY363014) is a rabbit polyclonal antibody detecting LPL/LIPD in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human LPL (His55-Glu316).
Cible Phospholipase A1, LIPD, Lipoprotein lipase, LPL
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès P06858
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Lipoprotein lipase (LPL/LIPD) is a ~53 kDa protein. Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.

1. Lutz, EP. et al. (2001) The Journal of clinical investigation 107, 1183-92. PMID: 11342582
2. Pingitore, P. et al. (2016) Journal of clinical lipidology 10, 816-823. PMID: 27578112
3. Weinstock, PH. et al. (1995) The Journal of clinical investigation 96, 2555-68. PMID: 8675619
4. McCoy, MG. et al. (2002) Journal of lipid research 43, 921-9. PMID: 12032167
5. Dugi, KA. et al. (1995) The Journal of biological chemistry 270, 25396-401. PMID: 7592706
6. Goulbourne, CN. et al. (2014) Cell metabolism 19, 849-60. PMID: 24726386
7. Allan, CM. et al. (2017) Journal of lipid research 58, 216-225. PMID: 27811232
Note For research use only.
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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