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Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Polyclonal Antibody (HC293014)

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Aperçu
Numéro de catalogueHC293014
Description
Anti-LpPLA2/PLA2G7/PAF-AH Polyclonal Antibody (HC293014) is a rabbit polyclonal antibody detecting PLA2G7 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human LpPLA2/PLA2G7/PAF-AH (Met33-Asn441).
Cible Platelet-activating factor acetylhydrolase, PAFAH, PAF 2-acylhydrolase, PAF acetylhydrolase, 2-acetyl-1-alkylglycerophosphocholine esterase, 1-alkyl-2-acetylglycerophosphocholine esterase, LDL-PLA(2), LDL-associated phospholipase A2, PLA2G7, Group-VIIA phospholipase A2, gVIIA-PLA2
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès Q13093
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Platelet-activating factor acetylhydrolase (Lp-PLA2/PLA2G7) is a ~50 kDa protein. Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells.

1. Stafforini, DM. et al. (1999) The Journal of biological chemistry 274, 7018-24. PMID: 10066756
2. Stafforini, DM. et al. (2006) The Journal of biological chemistry 281, 4616-23. PMID: 16371369
3. Kriska, T. et al. (2007) The Journal of biological chemistry 282, 100-8. PMID: 17090529
4. Stremler, KE. et al. (1991) The Journal of biological chemistry 266, 11095-103. PMID: 2040620
5. Tjoelker, LW. et al. (1995) Nature 374, 549-53. PMID: 7700381
6. Tew, DG. et al. (1996) Arteriosclerosis, thrombosis, and vascular biology 16, 591-9. PMID: 8624782
7. Min, JH. et al. (1999) Biochemistry 38, 12935-42. PMID: 10504265
9. Gardner, AA. et al. (2008) The Journal of biological chemistry 283, 17099-106. PMID: 18434304
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Polyclonal Antibody

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