Anti-MCM5 Polyclonal Antibody (HW592014)

Name: Anti-MCM5 Polyclonal Antibody
Brand: abinScience
SKU: HW592014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HW592014

Description

Anti-MCM5 Polyclonal Antibody (HW592014) is a rabbit polyclonal antibody detecting MCM5 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Xenopus tropicalis, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human MCM5 (Val331-Ile537).

Cible

CDC46, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46, MCM5

Purification

Purified by antigen affinity column.

Numéro d'accès

P33992

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

DNA replication licensing factor MCM5 is a ~82 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

1. Li, Z. et al. (2025) The EMBO journal 44, 6019-6050. PMID: 40940420

2. Tsuji, T. et al. (2006) Molecular biology of the cell 17, 4459-72. PMID: 16899510

3. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

4. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

5. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

6. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

Note

For research use only.

Images

Reférences

Fiche technique

Anti-MCM5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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