Human MCM5 Recombinant Protein (N-His) (HW592012)

Name: Human MCM5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW592012
Price: 211 USD
Availability: InStock

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Catalog No.	HW592012
Description	Recombinant Human MCM5 Protein, N-His (HW592012) expressed in E. coli, spanning Val331-Ile537. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P33992
Protein length	Val331-Ile537
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.76 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CDC46, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46, MCM5
Background	DNA replication licensing factor MCM5 is a ~82 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. 1. Li, Z. et al. (2025) The EMBO journal 44, 6019-6050. PMID: 40940420 2. Tsuji, T. et al. (2006) Molecular biology of the cell 17, 4459-72. PMID: 16899510 3. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425 4. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004 5. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328 6. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232
Note	For research use only.

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SDS-PAGE for Recombinant Human MCM5 Protein, N-His

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Datasheet

Human MCM5 Recombinant Protein (N-His).pdf

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Human MCM5 Recombinant Protein (N-His) (HW592012)

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