Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody (MW474014)

Name: Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody
Brand: abinScience
SKU: MW474014
Price: 96 USD
Availability: InStock

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Каталожный номер

MW474014

Описание

Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody (MW474014) is a rabbit polyclonal antibody detecting CASP4/Caspase-4 in ELISA, IHC, WB. Suitable for Mus musculus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Mouse CASP4/Caspase-4 (Pro81-Glu266).

Цель

CASP4/Caspase-4

Очистка

Purified by antigen affinity column.

Номер доступа

P70343

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Альтернативные названия

Caspase-4, CASP-4, 3.4.22.64, Caspase-11, CASP-11, Protease ICH-3, Caspase-4 subunit p10, Caspase-4 subunit p20, Casp4, Casp11, Caspl, Ich3

Фон

Caspase-4 (CASP4) is a ~42 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS and GSDMD. In contrast to its human ortholog, does not cleave IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B and IL18.

1. Kayagaki, N. et al. (2011) Nature 479, 117-21. PMID: 22002608

2. Aachoui, Y. et al. (2013) Science (New York, N.Y.) 339, 975-8. PMID: 23348507

3. Kayagaki, N. et al. (2013) Science (New York, N.Y.) 341, 1246-9. PMID: 23887873

4. Hagar, JA. et al. (2013) Science (New York, N.Y.) 341, 1250-3. PMID: 24031018

5. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034

6. Lee, BL. et al. (2018) The Journal of experimental medicine 215, 2279-2288. PMID: 30135078

7. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519

8. Wei, C. et al. (2024) Nature 629, 893-900. PMID: 38632402

9. Aachoui, Y. et al. (2015) Cell host & microbe 18, 320-32. PMID: 26320999

10. Hara, H. et al. (2018) Cell 175, 1651-1664.e14. PMID: 30392956

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-CASP4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: U2OS cell lysate

Ссылки

Даташит

Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody (MW474014)

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