ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Mus musculus (Mouse)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
23.42 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Caspase-4 is a ~42 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS and GSDMD. In contrast to its human ortholog, does not cleave IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B and IL18.
1. Kayagaki, N. et al. (2011) Nature 479, 117-21. PMID: 22002608
2. Aachoui, Y. et al. (2013) Science (New York, N.Y.) 339, 975-8. PMID: 23348507
3. Kayagaki, N. et al. (2013) Science (New York, N.Y.) 341, 1246-9. PMID: 23887873
4. Hagar, JA. et al. (2013) Science (New York, N.Y.) 341, 1250-3. PMID: 24031018
5. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034
6. Lee, BL. et al. (2018) The Journal of experimental medicine 215, 2279-2288. PMID: 30135078
7. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519
8. Wei, C. et al. (2024) Nature 629, 893-900. PMID: 38632402
9. Aachoui, Y. et al. (2015) Cell host & microbe 18, 320-32. PMID: 26320999
10. Hara, H. et al. (2018) Cell 175, 1651-1664.e14. PMID: 30392956
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Mouse CASP4/Caspase-4 Protein, N-His
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