Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody (MW474014)

Name: Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody
Brand: abinScience
SKU: MW474014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MW474014

Description

Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody (MW474014) is a rabbit polyclonal antibody detecting CASP4/Caspase-4 in ELISA, IHC, WB. Suitable for Mus musculus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CASP4/Caspase-4 (Pro81-Glu266).

Target

CASP4/Caspase-4

Purification

Purified by antigen affinity column.

Accession

P70343

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Caspase-4, CASP-4, 3.4.22.64, Caspase-11, CASP-11, Protease ICH-3, Caspase-4 subunit p10, Caspase-4 subunit p20, Casp4, Casp11, Caspl, Ich3

Background

Caspase-4 (CASP4) is a ~42 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS and GSDMD. In contrast to its human ortholog, does not cleave IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B and IL18.

1. Kayagaki, N. et al. (2011) Nature 479, 117-21. PMID: 22002608

2. Aachoui, Y. et al. (2013) Science (New York, N.Y.) 339, 975-8. PMID: 23348507

3. Kayagaki, N. et al. (2013) Science (New York, N.Y.) 341, 1246-9. PMID: 23887873

4. Hagar, JA. et al. (2013) Science (New York, N.Y.) 341, 1250-3. PMID: 24031018

5. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034

6. Lee, BL. et al. (2018) The Journal of experimental medicine 215, 2279-2288. PMID: 30135078

7. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519

8. Wei, C. et al. (2024) Nature 629, 893-900. PMID: 38632402

9. Aachoui, Y. et al. (2015) Cell host & microbe 18, 320-32. PMID: 26320999

10. Hara, H. et al. (2018) Cell 175, 1651-1664.e14. PMID: 30392956

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CASP4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: U2OS cell lysate

References

Datasheet

Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody (MW474014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us