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Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3) (MC336013)

Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3)
Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3)
Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3)
Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3)
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  • 20ug
  • 50ug
  • 100ug
  • 1mg
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Aperçu
Numéro de catalogueMC336013
Description
Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3) (MC336013) is a mouse monoclonal antibody detecting 4-1BB/TNFRSF9/CD137 in FCM, IF, ICC, ELISA, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesMouse
ApplicationsFCM, IF, ICC, ELISA, WB
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, kappa
ID de cloneLOB12.3
Applications testées WB
Cible 4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9
Niveau d'endotoxines < 10 EU/mg
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P20334
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Tumor necrosis factor receptor superfamily member 9 (CD137/4-1BB) is a ~27 kDa protein. Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors.

Note For research use only
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images
  • Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3)

    Western blot

    Western blot analysis was performed using anti-CD137 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant mouse CD137 (Catalog No: MC336012)
    Lane 2: negative control

  • Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3)

    Immunofluorescence

    Immunofluorescence staining of HDLM2 with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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g/mol
Formula
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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