Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (3H3) (MC336207)

Name: Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (3H3)
Brand: abinScience
SKU: MC336207
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	MC336207
Species reactivity	Mouse
Applications	in vivo 4-1BB stimulation, in vitro 4-1BB stimulation
Host species	Rat
Clonality	Monoclonal
Isotype	IgG2a, kappa
Clone ID	3H3
Tested applications	FCM
Target	4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9
Endotoxin level	< 10 EU/mg
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P20334
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Tumor necrosis factor receptor superfamily member 9 (CD137/4-1BB) is a ~27 kDa protein. Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors.
Note	For research use only.

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Bioactivity

Detects CD137/4-1BB/TNFRSF9 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-mouse CD137 antibody.CD137/4-1BB/TNFRSF9 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD137 antibody monoclonal antibody (Catalog # MC336207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.
Immunofluorescence

Immunofluorescence staining of HDLM2 with Rat monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.

References

Datasheet

Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (3H3).pdf

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Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (3H3) (MC336207)

Overview

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References

Datasheet

SDS

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