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Anti-Mouse CD16/CD32 Recombinant Antibody (2.4G2) (MP369107)

Anti-Mouse CD16/CD32 Recombinant Antibody (2.4G2)
Anti-Mouse CD16/CD32 Recombinant Antibody (2.4G2)
Anti-Mouse CD16/CD32 Recombinant Antibody (2.4G2)
Anti-Mouse CD16/CD32 Recombinant Antibody (2.4G2)
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Especificación:
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoMP369107
Reactividad de especiesMouse
AplicacionesFCM, FuncS, IF, IP
Especie huéspedRat
ClonalidadMonoclonal
IsotipoIgG2b, kappa
ID de clon2.4G2
Objetivo Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Lymphocyte antigen 17, Ly-17, CD32, Fcgr2, Fcgr2b, Ly-17
Nivel de endotoxinas <1 EU/mg
Pureza >95% as determined by SDS-PAGE.
Productos asociados pH 7.4 Dilution Buffer
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P08508, P08101
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Low affinity immunoglobulin gamma Fc region receptor III (CD16/CD32) is a ~30 kDa protein. Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b. Mediates neutrophil activation by IgG complexes redundantly with Fcgr4.

1. Hirano, M. et al. (2007) Nature immunology 8, 762-71. PMID: 17558411
2. Jakus, Z. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 618-29. PMID: 18097064
Nota For research use only.
Imágenes
  • Anti-Mouse CD16/CD32 Recombinant Antibody (2.4G2)

    Bioactivity

    Detects CD16/CD32 in indirect ELISAs.

  • Anti-Mouse CD16/CD32 Recombinant Antibody (2.4G2)

    Flow Cytometry

    Flow-cytometry using anti-mouse CD16 antibody.CD16 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD16 antibody monoclonal antibody (Catalog # MP369107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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