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| カタログ番号 | MB613013 | ||||
|---|---|---|---|---|---|
| 説明 |
Anti-Mouse CD223/LAG3 Nanobody (SAA0767) [SAA0767] (MB613013) is a alpaca monoclonal antibody detecting CD223 in ELISA, FCM. Suitable for Mouse.
Highlights
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| 種反応性 | Mouse | ||||
| アプリケーション | ELISA, FCM | ||||
| 宿主種 | Alpaca | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | VHH-8His-Cys-tag | ||||
| クローンID | SAA0767 | ||||
| ターゲット | CD223, FDC, LAG-3, LAG3, Lymphocyte activation gene 3 protein, Secreted lymphocyte activation gene 3 protein, sLAG-3 | ||||
| エンドトキシンレベル | < 10 EU/mg | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Purified by Nickel column. | ||||
| アクセッション番号 | Q61790 | ||||
| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 背景 | Lymphocyte activation gene 3 protein (CD223/LAG3) is a ~56 kDa protein. Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as MHC class II, its main ligand present at the surface of antigen-presenting cells (APCs), and FGL1, which is secreted by hepatocytes and certain types of tumor cells. Ligand-binding initiates a signaling that inhibits the T-cell receptor (TCR) in the immunological synapse, preventing T-cell activation. Mechanistically, ligand-binding promotes (1) ubiquitination of the KIEELE motif, unleashing the RRFSALE motif from the membrane and (2) leading to the formation of condensates with the TCR component CD3E, thereby disrupting the association between CD3E and LCK and preventing TCR activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1. CD223 is the therapeutic target of relatlimab (Opdualag). 1. Workman, CJ. et al. (2002) European journal of immunology 32, 2255-63. PMID: 12209638 2. Workman, CJ. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 169, 5392-5. PMID: 12421911 3. Workman, CJ. et al. (2003) European journal of immunology 33, 970-9. PMID: 12672063 4. Workman, CJ. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 5450-5. PMID: 15100286 5. Workman, CJ. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 174, 688-95. PMID: 15634887 6. Wang, J. et al. (2019) Cell 176, 334-347.e12. PMID: 30580966 7. Maruhashi, T. et al. (2022) Immunity 55, 912-924.e8. PMID: 35413245 8. Ming, Q. et al. (2022) Nature immunology 23, 1031-1041. PMID: 35761082 9. Silberstein, JL. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2310866121. PMID: 38483996 10. Ming, Q. et al. (2024) Nature communications 15, 7513. PMID: 39209860 | ||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Mouse CD223/LAG3 Nanobody (SAA0767)
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