Recombinant Mouse CD223/LAG3 Protein, C-His (MB613011) expressed in Mammalian Cells, spanning Gly24-Leu442. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Mammalian cells
Accession
Q61790
Protein length
Gly24-Leu442
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Mus musculus (Mouse)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
48.14 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Lymphocyte activation gene 3 protein is a ~56 kDa protein. Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as MHC class II, its main ligand present at the surface of antigen-presenting cells (APCs), and FGL1, which is secreted by hepatocytes and certain types of tumor cells. Ligand-binding initiates a signaling that inhibits the T-cell receptor (TCR) in the immunological synapse, preventing T-cell activation. Mechanistically, ligand-binding promotes (1) ubiquitination of the KIEELE motif, unleashing the RRFSALE motif from the membrane and (2) leading to the formation of condensates with the TCR component CD3E, thereby disrupting the association between CD3E and LCK and preventing TCR activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1.
1. Workman, CJ. et al. (2002) European journal of immunology 32, 2255-63. PMID: 12209638
2. Workman, CJ. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 169, 5392-5. PMID: 12421911
3. Workman, CJ. et al. (2003) European journal of immunology 33, 970-9. PMID: 12672063
4. Workman, CJ. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 5450-5. PMID: 15100286
5. Workman, CJ. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 174, 688-95. PMID: 15634887
6. Wang, J. et al. (2019) Cell 176, 334-347.e12. PMID: 30580966
7. Maruhashi, T. et al. (2022) Immunity 55, 912-924.e8. PMID: 35413245
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