Anti-Mouse CD47 Recombinant Antibody (A4), APC (MC359137)

Name: Anti-Mouse CD47 Recombinant Antibody (A4), APC
Brand: abinScience
SKU: MC359137
Price: 256 USD
Availability: InStock

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Каталожный номер	MC359137
Реактивность видов	Mouse
Применения	FCM
Хозяинский вид	Mouse
Клоональность	Monoclonal
Изотип	VHH, mFc
ID клона	A4
Конъюгация	APC
Проверенные приложения	FCM
Цель	Antigenic surface determinant protein OA3, CD47, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Связанные продукты	VHH-mFc Isotype Control antibody (cAb-Lys2), APC
Номер доступа	Q61735
Форма	Liquid
Буфер хранения	0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон	Leukocyte surface antigen CD47 (MER6) is a ~33 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical). 1. Bauer, EM. et al. (2010) Cardiovascular research 88, 471-81. PMID: 20610415 2. Kaur, S. et al. (2013) Scientific reports 3, 1673. PMID: 23591719 3. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621 4. Manna, PP. et al. (2005) The Journal of biological chemistry 280, 29637-44. PMID: 15917238 5. Ghimire, K. et al. (2020) Cells 9. PMID: 32679764 6. Isenberg, JS. et al. (2008) Annals of surgery 247, 180-90. PMID: 18156939 7. Oldenborg, PA. et al. (2000) Science (New York, N.Y.) 288, 2051-4. PMID: 10856220
Примечание	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using APC anti-Mouse CD47 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-Mouse CD47 monoclonal antibody (Catalog: MC359137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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