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Anti-Mouse CD47 Recombinant Antibody (A4) (MC359107)

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Anti-Mouse CD47 Recombinant Antibody (A4)

Anti-Mouse CD47 Recombinant Antibody (A4)

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Overview

Catalog No.	MC359107
Description	Anti-Mouse CD47/MER6 Antibody (A4) [A4] (MC359107) is a mouse monoclonal antibody detecting CD47 in FCM. Suitable for Mouse. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Mouse
Applications	FCM
Host species	Mouse
Isotype	VHH-mFc
Clone ID	A4
Clonality	Monoclonal
Target	Leukocyte surface antigen CD47, Integrin-associated protein, IAP, CD47
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q61735
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Leukocyte surface antigen CD47 (MER6) is a ~33 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical). 1. Bauer, EM. et al. (2010) Cardiovascular research 88, 471-81. PMID: 20610415 2. Kaur, S. et al. (2013) Scientific reports 3, 1673. PMID: 23591719 3. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621 4. Manna, PP. et al. (2005) The Journal of biological chemistry 280, 29637-44. PMID: 15917238 5. Ghimire, K. et al. (2020) Cells 9. PMID: 32679764 6. Isenberg, JS. et al. (2008) Annals of surgery 247, 180-90. PMID: 18156939 7. Oldenborg, PA. et al. (2000) Science (New York, N.Y.) 288, 2051-4. PMID: 10856220
Note	For research use only.

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Western blot

Western blot analysis was performed using anti-CD47/IAP monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant mouse CD47/IAP (Catalog No: MC359011)
Lane 2: negative control

References

Datasheet

Anti-Mouse CD47 Recombinant Antibody (A4).pdf

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Anti-Mouse CD47 Recombinant Antibody (A4)