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Anti-Mouse CD5 Polyclonal Antibody (MY046014)

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Aperçu
Numéro de catalogueMY046014
Description
Anti-Mouse CD5 Polyclonal Antibody (MY046014) is a rabbit polyclonal antibody detecting T-cell surface glycoprotein CD5; Lymphocyte antigen 1; Ly-1; Lyt-1; CD5; Cd5; Ly-1 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesMouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant mouse CD5 (Gln24-Pro371).
Cible T-cell surface glycoprotein CD5; Lymphocyte antigen 1; Ly-1; Lyt-1; CD5; Cd5; Ly-1
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P13379
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

T-cell surface glycoprotein CD5 is a ~53 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation.

1. Schuster, C. et al. (2022) Frontiers in immunology 13, 906499. PMID: 35720357
2. Zheng, M. et al. (2014) Immunological investigations 43, 705-16. PMID: 24950378
3. Biancone, L. et al. (1996) The Journal of experimental medicine 184, 811-9. PMID: 9064341
4. Bikah, G. et al. (1998) International immunology 10, 1185-96. PMID: 9723705
5. Dennehy, KM. et al. (2001) International immunology 13, 149-56. PMID: 11157848
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse CD5 Polyclonal Antibody

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