Anti-Mouse CD5/Ly-1 Recombinant Antibody (SAA2083) (MY046013)

Name: Anti-Mouse CD5/Ly-1 Recombinant Antibody (SAA2083)
Brand: abinScience
SKU: MY046013
Price: 110 USD
Availability: InStock

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Spec:

20ug
50ug
100ug
1mg

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MY046013

Species reactivity

Mouse

Applications

ELISA

Host species

Human

Isotype

VHH-hFc

Clone ID

SAA2083

Clonality

Monoclonal

Target

Lyt-1, Cd5, CD5, T-cell surface glycoprotein CD5, Ly-1, Lymphocyte antigen 1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P13379

RRID

Anti-Mouse CD5/Ly-1 Antibody (SAA2083) (abinScience Cat# MY046013, RRID:AB_3729343)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

T-cell surface glycoprotein CD5 (Ly-1) is a ~53 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation.

1. Schuster, C. et al. (2022) Frontiers in immunology 13, 906499. PMID: 35720357

2. Zheng, M. et al. (2014) Immunological investigations 43, 705-16. PMID: 24950378

3. Biancone, L. et al. (1996) The Journal of experimental medicine 184, 811-9. PMID: 9064341

4. Bikah, G. et al. (1998) International immunology 10, 1185-96. PMID: 9723705

5. Dennehy, KM. et al. (2001) International immunology 13, 149-56. PMID: 11157848

Note

For research use only.

Images

Bioactivity

Detects Recombinant Mouse CD5 Protein, C-His (Cat No.: MY046011) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Anti-Mouse CD5/Ly-1 Antibody (SAA2083)