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Mouse CD5 Recombinant Protein (N-His) (MY046012)

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Mouse CD5 Recombinant Protein (N-His)

Mouse CD5 Recombinant Protein (N-His)

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Overview

Catalog No.	MY046012
Description	Recombinant Mouse CD5 Protein, N-His (MY046012) expressed in E. coli, spanning Gln24-Pro371. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P13379
Protein length	Gln24-Pro371
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	40.22 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	T-cell surface glycoprotein CD5; Lymphocyte antigen 1; Ly-1; Lyt-1; CD5; Cd5; Ly-1
Background	T-cell surface glycoprotein CD5 is a ~53 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. 1. Schuster, C. et al. (2022) Frontiers in immunology 13, 906499. PMID: 35720357 2. Zheng, M. et al. (2014) Immunological investigations 43, 705-16. PMID: 24950378 3. Biancone, L. et al. (1996) The Journal of experimental medicine 184, 811-9. PMID: 9064341 4. Bikah, G. et al. (1998) International immunology 10, 1185-96. PMID: 9723705 5. Dennehy, KM. et al. (2001) International immunology 13, 149-56. PMID: 11157848
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Mouse CD5 Protein, N-His

References

Datasheet

Mouse CD5 Recombinant Protein (N-His).pdf

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Formula

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Mouse CD5 Recombinant Protein (N-His)