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Anti-Mouse CD83 Polyclonal Antibody (MX237014)

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Anti-Mouse CD83 Polyclonal Antibody
Anti-Mouse CD83 Polyclonal Antibody
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Aperçu
Numéro de catalogueMX237014
Description
Anti-Mouse CD83 Polyclonal Antibody (MX237014) is a rabbit polyclonal antibody detecting CD83 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesMouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Mouse CD83 (Met22-Cys120).
Applications testées WB
Cible CD83, hCD83, CD83 antigen, B-cell activation protein, Cell surface protein HB15
Purification Purified by antigen affinity column.
Numéro d'accès O88324
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

CD83 antigen is a ~21 kDa protein. Transmembrane glycoprotein predominantly found on the surface of many immune cells including dendritic cells or lymphocytes that plays various roles in immune response regulation. Plays an essential role in CD4(+) T-selection, differentiation and stability by regulating the activity of the major E3 ubiquitin ligase responsible for controlling MHC II trafficking MARCHF8. Also inhibits MARCHF1 association with MHC II and ubiquitination of MHCII. In addition, acts as an important modulator of protective responses against acute infections.

1. von Rohrscheidt, J. et al. (2016) The Journal of experimental medicine 213, 1685-94. PMID: 27503071
2. Liu, H. et al. (2016) The Journal of experimental medicine 213, 1695-703. PMID: 27503069
3. Wild, AB. et al. (2019) JCI insight 4. PMID: 31527313
Note For research use only.
Images
  • Anti-Mouse CD83 Polyclonal Antibody

    Western blot

    Lane 1: Hela cell lysate

Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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