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| カタログ番号 | MX011013 | ||||
|---|---|---|---|---|---|
| 種反応性 | Mouse | ||||
| アプリケーション | ELISA, FCM | ||||
| 宿主種 | Rat | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG2b, kappa | ||||
| クローンID | JES6-1A12 | ||||
| テスト済みアプリケーション | WB | ||||
| ターゲット | IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | P04351 | ||||
| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | Interleukin-2 (IL2) is a ~19 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. 1. Zhu, J. et al. (2003) Immunity 19, 739-48. PMID: 14614860 2. Reya, T. et al. (1998) Developmental immunology 5, 287-302. PMID: 9814585 3. Smith, GA. et al. (2016) Nature chemical biology 12, 373-9. PMID: 27018889 | ||||
| 注意事項 | For research use only. | ||||
| Isotype Control | Rat IgG2b Recombinant Isotype Control Antibody (LTF-2) (RF974107) |
Detects IL2 in indirect ELISAs.
SDS-PAGE for Anti-Mouse IL2 Antibody (JES6-1A12).
Western blot analysis was performed using anti-IL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant mouse IL2 (Catalog No: MX011012)
Lane 2: negative control
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