Anti-Mouse IL-2 Polyclonal Antibody (MX011014)

Name: Anti-Mouse IL-2 Polyclonal Antibody
Brand: abinScience
SKU: MX011014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

MX011014

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse IL2 (Ala21-Gln169).

Target

IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor

Purification

Purified by antigen affinity column.

Accession

P04351

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-2 (IL2) is a ~19 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways.

1. Zhu, J. et al. (2003) Immunity 19, 739-48. PMID: 14614860

2. Reya, T. et al. (1998) Developmental immunology 5, 287-302. PMID: 9814585

3. Smith, GA. et al. (2016) Nature chemical biology 12, 373-9. PMID: 27018889

Note

For research use only.

Images

References

Datasheet

Anti-Mouse IL-2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse IL-2 Polyclonal Antibody (MX011014)

Overview

Images

References

Datasheet

SDS

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