Anti-Mouse IL-2 Recombinant Antibody (JES6-1A12) (MX011013)

Name: Anti-Mouse IL-2 Recombinant Antibody (JES6-1A12)
Brand: abinScience
SKU: MX011013
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MX011013

Species reactivity

Mouse

Applications

ELISA, FCM

Host species

Rat

Isotype

IgG2b, kappa

Clone ID

JES6-1A12

Clonality

Monoclonal

Tested applications

Target

IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P04351

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Interleukin-2 (IL2) is a ~19 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways.

1. Zhu, J. et al. (2003) Immunity 19, 739-48. PMID: 14614860

2. Reya, T. et al. (1998) Developmental immunology 5, 287-302. PMID: 9814585

3. Smith, GA. et al. (2016) Nature chemical biology 12, 373-9. PMID: 27018889

Note

For research use only.

Images

Bioactivity

Detects IL2 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Mouse IL2 Antibody (JES6-1A12).
Western blot

Western blot analysis was performed using anti-IL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant mouse IL2 (Catalog No: MX011012)
Lane 2: negative control