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Anti-Mouse IL-9 Polyclonal Antibody (MB771014)

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Aperçu
Numéro de catalogueMB771014
Description
Anti-Mouse IL9 Polyclonal Antibody (MB771014) is a rabbit polyclonal antibody detecting Il9. Suitable for Mouse.
Reactivité des espècesMouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Mouse IL9 (Gln19-Pro144).
Cible Cytokine P40, IL-9, Il9, Interleukin-9, T-cell growth factor P40
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès P15247
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Interleukin-9 (IL9) is a ~16 kDa protein. Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites. Affects intestinal epithelial permeability and adaptive immunity. In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells. Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG. Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs.

1. Townsend, JM. et al. (2000) Immunity 13, 573-83. PMID: 11070175
2. Elyaman, W. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 12885-90. PMID: 19433802
3. Khan, WI. et al. (2003) Infection and immunity 71, 2430-8. PMID: 12704113
4. Druez, C. et al. (1990) Journal of immunology (Baltimore, Md. : 1950) 145, 2494-9. PMID: 2145361
5. Kimura, Y. et al. (1995) International immunology 7, 115-20. PMID: 7718508
6. Demoulin, JB. et al. (1999) The Journal of biological chemistry 274, 25855-61. PMID: 10464327
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Molecular Weight *
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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