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Anti-Mouse MAG/Myelin-associated glycoprotein Polyclonal Antibody (MA497014)

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Обзор
Каталожный номерMA497014
Описание
Anti-Mouse MAG/Myelin-associated glycoprotein Polyclonal Antibody (MA497014) is a rabbit polyclonal antibody detecting MAG/Myelin-associated glycoprotein in ELISA, IHC, WB. Suitable for Rattus norvegicus, Human, and Mus musculus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Mouse MAG/Myelin-associated glycoprotein (Asp235-Glu410).
Цель MAG/Myelin-associated glycoprotein
Очистка Purified by antigen affinity column.
Номер доступа P20917
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияMyelin-associated glycoprotein, Siglec-4a, Mag
Фон

Myelin-associated glycoprotein (MAG) is a ~69 kDa protein. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2. Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane. Negative regulator of neurite outgrowth that inhibits axon longitudinal growth.

1. Liu, BP. et al. (2002) Science (New York, N.Y.) 297, 1190-3. PMID: 12089450
2. Pronker, MF. et al. (2016) Nature communications 7, 13584. PMID: 27922006
3. Kelm, S. et al. (1994) Current biology : CB 4, 965-72. PMID: 7533044
4. Schachner, M. et al. (2000) Glia 29, 154-65. PMID: 10625334
5. Li, C. et al. (1994) Nature 369, 747-50. PMID: 7516497
6. Bartsch, S. et al. (1997) Brain research 762, 231-4. PMID: 9262180
7. Li, C. et al. (1998) Journal of neuroscience research 51, 210-7. PMID: 9469574
8. Yin, X. et al. (1998) The Journal of neuroscience : the official journal of the Society for Neuroscience 18, 1953-62. PMID: 9482781
9. Fujita, N. et al. (1998) The Journal of neuroscience : the official journal of the Society for Neuroscience 18, 1970-8. PMID: 9482783
10. Palandri, A. et al. (2015) Cell death & disease 6, e1876. PMID: 26335717
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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