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| カタログ番号 | MB945023 | ||||
|---|---|---|---|---|---|
| 種反応性 | Human, Mouse, Rat | ||||
| アプリケーション | FCM, IHC, IP, WB | ||||
| 宿主種 | Mouse | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG2a, kappa | ||||
| クローンID | L51/82 | ||||
| テスト済みアプリケーション | FCM | ||||
| ターゲット | Matrix metalloproteinase-9, CLG4B, GELB, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, MMP9, MMP-9 | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | P41245 | ||||
| RRID | Anti-Mouse MMP9 Antibody (L51/82) (abinScience Cat# MB945023, RRID:AB_3725634) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | Matrix metalloproteinase-9 (MMP9) is a ~80 kDa protein. Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. 1. Reponen, P. et al. (1994) The Journal of cell biology 124, 1091-1102. PMID: 8132709 2. Ahn, BJ. et al. (2012) Biochemical and biophysical research communications 428, 438-44. PMID: 23142597 3. Jeon, S. et al. (2020) Circulation 142, 1736-1751. PMID: 32883094 | ||||
| 注意事項 | For research use only. | ||||
| Isotype Control | Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507) Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307) |
Flow-cytometry using anti-mouse MMP9 antibody.MMP9 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse MMP9 antibody monoclonal antibody (Catalog # MB945023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Mouse MMP9 Antibody (L51/82).
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