Anti-Mouse MMP-9 Recombinant Antibody (SDS3) (MB945013)

Name: Anti-Mouse MMP-9 Recombinant Antibody (SDS3)
Brand: abinScience
SKU: MB945013
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MB945013

Species reactivity

Human, Mouse

Applications

ELISA, IP

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SDS3

Clonality

Monoclonal

Tested applications

FCM

Target

Matrix metalloproteinase-9, CLG4B, GELB, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, MMP9, MMP-9

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P41245

RRID

Anti-Mouse MMP9 Antibody (SDS3) (abinScience Cat# MB945013, RRID:AB_3725633)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Matrix metalloproteinase-9 (MMP9) is a ~80 kDa protein. Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments.

1. Reponen, P. et al. (1994) The Journal of cell biology 124, 1091-1102. PMID: 8132709

2. Ahn, BJ. et al. (2012) Biochemical and biophysical research communications 428, 438-44. PMID: 23142597

3. Jeon, S. et al. (2020) Circulation 142, 1736-1751. PMID: 32883094

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse MMP9 Antibody (SDS3).
Flow CytoMetry

Flow-cytometry using PE anti-human MMP2 antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human MMP2 monoclonal antibody (Catalog: MB945013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human MMP9 antibody. BxPC-3 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human MMP9 monoclonal antibody (Catalog: MB945013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.