





| Каталожный номер | MB945013 | ||||
|---|---|---|---|---|---|
| Реактивность видов | Human, Mouse | ||||
| Применения | ELISA, IP | ||||
| Хозяинский вид | Mouse | ||||
| Клоональность | Monoclonal | ||||
| Изотип | IgG2a, kappa | ||||
| ID клона | SDS3 | ||||
| Проверенные приложения | FCM | ||||
| Цель | Matrix metalloproteinase-9, CLG4B, GELB, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, MMP9, MMP-9 | ||||
| Уровень эндотоксинов | Please contact with the lab for this information. | ||||
| Чистота | >95% as determined by SDS-PAGE. | ||||
| Очистка | Protein A/G purified from cell culture supernatant. | ||||
| Номер доступа | P41245 | ||||
| RRID | Anti-Mouse MMP9 Antibody (SDS3) (abinScience Cat# MB945013, RRID:AB_3725633) |
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| Форма | Liquid | ||||
| Буфер хранения | 0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
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| Информация об использовании продукта |
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| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Фон | Matrix metalloproteinase-9 (MMP9) is a ~80 kDa protein. Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. 1. Reponen, P. et al. (1994) The Journal of cell biology 124, 1091-1102. PMID: 8132709 2. Ahn, BJ. et al. (2012) Biochemical and biophysical research communications 428, 438-44. PMID: 23142597 3. Jeon, S. et al. (2020) Circulation 142, 1736-1751. PMID: 32883094 | ||||
| Примечание | For research use only. | ||||
| Isotype Control | Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307) |

SDS-PAGE for Anti-Mouse MMP9 Antibody (SDS3).

Flow-cytometry using PE anti-human MMP2 antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human MMP2 monoclonal antibody (Catalog: MB945013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human MMP9 antibody. BxPC-3 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human MMP9 monoclonal antibody (Catalog: MB945013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.




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