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Anti-Mouse UNG Polyclonal Antibody (MY404014)

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Aperçu
Numéro de catalogueMY404014
Description
Anti-Mouse UNG Polyclonal Antibody (MY404014) is a rabbit polyclonal antibody detecting UNG. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesMouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Mouse UNG (Ser60-Leu306).
Cible DGU, EC:3.2.2.27, UDG, UNG, UNG1, UNG15, Uracil-DNA glycosylase
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P97931
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Uracil-DNA glycosylase (UNG) is a ~33 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication.

1. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144
2. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624
3. Rada, C. et al. (2002) Current biology : CB 12, 1748-55. PMID: 12401169
4. Maul, RW. et al. (2011) Nature immunology 12, 70-6. PMID: 21151102
5. Schrader, CE. et al. (2005) The Journal of experimental medicine 202, 561-8. PMID: 16103411
Note For research use only
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Formula
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Formula
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