Mouse UNG Recombinant Protein (N-His) (MY404012)

Name: Mouse UNG Recombinant Protein (N-His)
Brand: abinScience
SKU: MY404012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MY404012
Description	Recombinant Mouse UNG Protein, N-His (MY404012) expressed in E. coli, spanning Ser60-Leu306. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P97931
Protein length	Ser60-Leu306
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	30.14 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DGU, EC:3.2.2.27, UDG, UNG, UNG1, UNG15, Uracil-DNA glycosylase
Background	Uracil-DNA glycosylase is a ~33 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication. 1. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144 2. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624 3. Rada, C. et al. (2002) Current biology : CB 12, 1748-55. PMID: 12401169 4. Maul, RW. et al. (2011) Nature immunology 12, 70-6. PMID: 21151102 5. Schrader, CE. et al. (2005) The Journal of experimental medicine 202, 561-8. PMID: 16103411
Note	For research use only

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Mouse UNG Recombinant Protein (N-His).pdf

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Mouse UNG Recombinant Protein (N-His) (MY404012)

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