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Anti-MPEG1 Polyclonal Antibody (HS702014)

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Aperçu
Numéro de catalogueHS702014
Description
Anti-MPEG1 Polyclonal Antibody (HS702014) is a rabbit polyclonal antibody detecting MPEG1 in ELISA, IHC, WB. Suitable for Human and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human MPEG1 (Phe313-Ser580).
Cible Macrophage-expressed gene 1 protein, Perforin-2, Mpg-1, MPEG1, P-2, Macrophage gene 1 protein
Purification Purified by antigen affinity column.
Numéro d'accès Q2M385
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Macrophage-expressed gene 1 protein (MPEG1) is a ~78 kDa protein. Pore-forming protein involved in both innate and adaptive immunity. Plays a central role in antigen cross-presentation in dendritic cells by forming a pore in antigen-containing compartments, thereby promoting delivery of antigens for cross-presentation. Also involved in innate immune response following bacterial infection; shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria. Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol. Required for the antibacterial activity of reactive oxygen species and nitric oxide.

1. Fields, KA. et al. (2013) Infection and immunity 81, 3045-54. PMID: 23753625
2. McCormack, RM. et al. (2015) eLife 4. PMID: 26402460
3. McCormack, RM. et al. (2017) JCI insight 2. PMID: 28422754
4. Strbo, N. et al. (2019) Experimental dermatology 28, 225-232. PMID: 30609079
5. Pang, SS. et al. (2019) Nature communications 10, 4288. PMID: 31537793
6. Merselis, LC. et al. (2020) Frontiers in immunology 11, 601584. PMID: 33224153
Note For research use only.
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-MPEG1 Polyclonal Antibody

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