Human MPEG1 Recombinant Protein (N-His) (HS702012)

Name: Human MPEG1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HS702012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HS702012
Description	Recombinant Human MPEG1 Protein, N-His (HS702012) expressed in E. coli, spanning Phe313-Ser580. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q2M385
Protein length	Phe313-Ser580
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	31.67 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Macrophage-expressed gene 1 protein, Perforin-2, Mpg-1, MPEG1, P-2, Macrophage gene 1 protein
Background	Macrophage-expressed gene 1 protein (MPEG1) is a ~78 kDa protein. Pore-forming protein involved in both innate and adaptive immunity. Plays a central role in antigen cross-presentation in dendritic cells by forming a pore in antigen-containing compartments, thereby promoting delivery of antigens for cross-presentation. Also involved in innate immune response following bacterial infection; shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria. Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol. Required for the antibacterial activity of reactive oxygen species and nitric oxide. 1. Fields, KA. et al. (2013) Infection and immunity 81, 3045-54. PMID: 23753625 2. McCormack, RM. et al. (2015) eLife 4. PMID: 26402460 3. McCormack, RM. et al. (2017) JCI insight 2. PMID: 28422754 4. Strbo, N. et al. (2019) Experimental dermatology 28, 225-232. PMID: 30609079 5. Pang, SS. et al. (2019) Nature communications 10, 4288. PMID: 31537793 6. Merselis, LC. et al. (2020) Frontiers in immunology 11, 601584. PMID: 33224153
Note	For research use only.

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SDS-PAGE for Recombinant Human MPEG1 Protein, N-His

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Datasheet

Human MPEG1 Recombinant Protein (N-His).pdf

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Human MPEG1 Recombinant Protein (N-His) (HS702012)

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