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Обзор
Каталожный номерHS702014
Описание
Anti-MPEG1 Polyclonal Antibody (HS702014) is a rabbit polyclonal antibody detecting MPEG1 in ELISA, IHC, WB. Suitable for Human and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human MPEG1 (Phe313-Ser580).
Цель Macrophage-expressed gene 1 protein, Perforin-2, Mpg-1, MPEG1, P-2, Macrophage gene 1 protein
Очистка Purified by antigen affinity column.
Номер доступа Q2M385
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Macrophage-expressed gene 1 protein (MPEG1) is a ~78 kDa protein. Pore-forming protein involved in both innate and adaptive immunity. Plays a central role in antigen cross-presentation in dendritic cells by forming a pore in antigen-containing compartments, thereby promoting delivery of antigens for cross-presentation. Also involved in innate immune response following bacterial infection; shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria. Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol. Required for the antibacterial activity of reactive oxygen species and nitric oxide.

1. Fields, KA. et al. (2013) Infection and immunity 81, 3045-54. PMID: 23753625
2. McCormack, RM. et al. (2015) eLife 4. PMID: 26402460
3. McCormack, RM. et al. (2017) JCI insight 2. PMID: 28422754
4. Strbo, N. et al. (2019) Experimental dermatology 28, 225-232. PMID: 30609079
5. Pang, SS. et al. (2019) Nature communications 10, 4288. PMID: 31537793
6. Merselis, LC. et al. (2020) Frontiers in immunology 11, 601584. PMID: 33224153
Примечание For research use only.
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Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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