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Anti-MSN Polyclonal Antibody (HB033014)

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Vista general
Número de catálogoHB033014
Descripción
Anti-MSN Polyclonal Antibody (HB033014) is a rabbit polyclonal antibody detecting MSN in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human MSN (Ile354-Met577).
Objetivo Membrane-organizing extension spike protein, MSN, Moesin
Purificación Purified by antigen affinity column.
Número de acceso P26038
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Moesin (MSN) is a ~67 kDa protein. Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton. Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement. These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction. The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs.

1. Huang, L. et al. (1999) The Journal of biological chemistry 274, 12803-10. PMID: 10212266
2. Urzainqui, A. et al. (2002) Immunity 17, 401-12. PMID: 12387735
3. Iontcheva, I. et al. (2004) Infection and immunity 72, 2312-20. PMID: 15039356
4. Serrador, JM. et al. (1997) The Journal of cell biology 138, 1409-23. PMID: 9298994
5. Serrador, JM. et al. (1998) Blood 91, 4632-44. PMID: 9616160
6. Lagresle-Peyrou, C. et al. (2016) The Journal of allergy and clinical immunology 138, 1681-1689.e8. PMID: 27405666
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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