Human MSN Recombinant Protein (N-His) (HB033012)

Name: Human MSN Recombinant Protein (N-His)
Brand: abinScience
SKU: HB033012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB033012
Description	Recombinant Human MSN Protein, N-His (HB033012) expressed in E. coli, spanning Ile354-Met577. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P26038
Protein length	Ile354-Met577
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.24 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Membrane-organizing extension spike protein, MSN, Moesin
Background	Moesin (MSN) is a ~67 kDa protein. Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton. Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement. These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction. The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs. 1. Huang, L. et al. (1999) The Journal of biological chemistry 274, 12803-10. PMID: 10212266 2. Urzainqui, A. et al. (2002) Immunity 17, 401-12. PMID: 12387735 3. Iontcheva, I. et al. (2004) Infection and immunity 72, 2312-20. PMID: 15039356 4. Serrador, JM. et al. (1997) The Journal of cell biology 138, 1409-23. PMID: 9298994 5. Serrador, JM. et al. (1998) Blood 91, 4632-44. PMID: 9616160 6. Lagresle-Peyrou, C. et al. (2016) The Journal of allergy and clinical immunology 138, 1681-1689.e8. PMID: 27405666
Note	For research use only.

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SDS-PAGE for Recombinant Human MSN Protein, N-His

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Datasheet

Human MSN Recombinant Protein (N-His).pdf

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Human MSN Recombinant Protein (N-His) (HB033012)

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