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Anti-Murine minute virus/MVM NS1 Polyclonal Antibody (VK011034)

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Aperçu
Numéro de catalogueVK011034
Description
Anti-Murine minute virus/MVM NS1 Polyclonal Antibody (VK011034) is a rabbit polyclonal antibody detecting NS1 in ELISA, IHC, WB. Suitable for Murine minute virus and Parvovirus LuIII.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesMurine minute virus (MVM)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Murine minute virus NS1 (Tyr6-Thr253).
Cible NS1
Pureté >90%
Purification Purified by antigen affinity column.
Numéro d'accès P03134
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsInitiator protein NS1, NS1, EC:3.1.21.-, EC:3.6.4.12, NCVP1, Non-capsid protein NS-1, Non-structural protein NS1, NS1
Contexte

Initiator protein NS1 is a ~76 kDa protein. Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies of the tetranucleotide 5'TGGT3' within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Cooperatively binds Ori with host PIF and probably other host factors, which activate the nickase function of NS1. Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis.

1. Christensen, J. et al. (2002) Journal of virology 76, 6518-31. PMID: 12050365
2. Christensen, J. et al. (1995) Journal of virology 69, 5422-30. PMID: 7636987
3. Cotmore, SF. et al. (2007) Journal of virology 81, 13015-27. PMID: 17898054
4. Willwand, K. et al. (1997) The Journal of general virology 78 ( Pt 10), 2647-55. PMID: 9349487
5. Christensen, J. et al. (2001) Journal of virology 75, 7009-17. PMID: 11435581
Note For research use only.
Images
Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Murine minute virus/MVM NS1 Polyclonal Antibody

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