Murine minute virus/MVM NS1 Recombinant Protein (N-His) (VK011032)

Name: Murine minute virus/MVM NS1 Recombinant Protein (N-His)
Brand: abinScience
SKU: VK011032
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK011032
Description	Recombinant Murine minute virus/MVM NS1 Protein, N-His (VK011032) expressed in E. coli, spanning Tyr6-Thr253. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P03134
Protein length	Tyr6-Thr253
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Murine minute virus (MVM)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	31.62 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Initiator protein NS1, NS1, EC:3.1.21.-, EC:3.6.4.12, NCVP1, Non-capsid protein NS-1, Non-structural protein NS1, NS1
Background	Initiator protein NS1 is a ~76 kDa protein. Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies of the tetranucleotide 5'TGGT3' within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Cooperatively binds Ori with host PIF and probably other host factors, which activate the nickase function of NS1. Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis. 1. Christensen, J. et al. (2002) Journal of virology 76, 6518-31. PMID: 12050365 2. Christensen, J. et al. (1995) Journal of virology 69, 5422-30. PMID: 7636987 3. Cotmore, SF. et al. (2007) Journal of virology 81, 13015-27. PMID: 17898054 4. Willwand, K. et al. (1997) The Journal of general virology 78 ( Pt 10), 2647-55. PMID: 9349487 5. Christensen, J. et al. (2001) Journal of virology 75, 7009-17. PMID: 11435581
Note	For research use only.

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SDS-PAGE for Recombinant Murine minute virus/MVM NS1 Protein, N-His

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Datasheet

Murine minute virus/MVM NS1 Recombinant Protein (N-His).pdf

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Murine minute virus/MVM NS1 Recombinant Protein (N-His) (VK011032)

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