Anti-Mycobacterium tuberculosis esxA/ESAT-6 Recombinant Antibody (TA005) (JN009033)

Name: Anti-Mycobacterium tuberculosis esxA/ESAT-6 Recombinant Antibody (TA005)
Brand: abinScience
SKU: JN009033
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN009033

Reactividad de especies

Mycobacterium tuberculosis

Aplicaciones

ELISA

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG1

ID de clon

TA005

Objetivo

6 kDa early secretory antigenic target, ESAT-6, esxA, esaT6, Rv3875, MTV027.10

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P9WNK7

RRID

Anti-Mycobacterium tuberculosis esxA/ESAT-6 Antibody (TA005) (abinScience Cat# JN009033, RRID:AB_3729540)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

6 kDa early secretory antigenic target (esxA/ESAT-6) is a ~9 kDa protein. A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL-12 p40 (IL12B) and TNF expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protective immune response. Higher levels (1.6-3.3 uM) of recombinant protein inhibit IFN-gamma production by host (human) T-cells and also IL-17 and TNF production but not IL-2; decreases expression of host ATF-2 and JUN transcription factors by affecting T-cell receptors signaling downstream of ZAP70, without cytotoxicity or apoptosis.

1. Renshaw, PS. et al. (2002) The Journal of biological chemistry 277, 21598-603. PMID: 11940590

2. Sørensen, AL. et al. (1995) Infection and immunity 63, 1710-7. PMID: 7729876

3. Stanley, SA. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 13001-6. PMID: 14557536

4. Andersen, P. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 3359-72. PMID: 7897219

5. Wang, X. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 3668-77. PMID: 19265145

6. Peng, H. et al. (2011) The Journal of biological chemistry 286, 24508-18. PMID: 21586573

7. Pathak, SK. et al. (2007) Nature immunology 8, 610-8. PMID: 17486091

8. Mishra, BB. et al. (2010) Cellular microbiology 12, 1046-63. PMID: 20148899

9. Boggaram, V. et al. (2013) The Journal of biological chemistry 288, 25500-25511. PMID: 23867456

10. Sreejit, G. et al. (2014) PLoS pathogens 10, e1004446. PMID: 25356553

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Mycobacterium tuberculosis esxA/ESAT-6 Antibody (TA005)

Referencias

Datasheet

Anti-Mycobacterium tuberculosis esxA/ESAT-6 Recombinant Antibody (TA005).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo