Anti-Mycobacterium tuberculosis esxA/ESAT-6 Recombinant Antibody (TA005) (JN009033)

Name: Anti-Mycobacterium tuberculosis esxA/ESAT-6 Recombinant Antibody (TA005)
Brand: abinScience
SKU: JN009033
Price: 126 USD
Availability: InStock

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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN009033

Reactivité des espèces

Mycobacterium tuberculosis

Applications

ELISA

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1

ID de clone

TA005

Cible

6 kDa early secretory antigenic target, ESAT-6, esxA, esaT6, Rv3875, MTV027.10

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P9WNK7

RRID

Anti-Mycobacterium tuberculosis esxA/ESAT-6 Antibody (TA005) (abinScience Cat# JN009033, RRID:AB_3729540)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

6 kDa early secretory antigenic target (esxA/ESAT-6) is a ~9 kDa protein. A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL-12 p40 (IL12B) and TNF expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protective immune response. Higher levels (1.6-3.3 uM) of recombinant protein inhibit IFN-gamma production by host (human) T-cells and also IL-17 and TNF production but not IL-2; decreases expression of host ATF-2 and JUN transcription factors by affecting T-cell receptors signaling downstream of ZAP70, without cytotoxicity or apoptosis.

1. Renshaw, PS. et al. (2002) The Journal of biological chemistry 277, 21598-603. PMID: 11940590

2. Sørensen, AL. et al. (1995) Infection and immunity 63, 1710-7. PMID: 7729876

3. Stanley, SA. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 13001-6. PMID: 14557536

4. Andersen, P. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 3359-72. PMID: 7897219

5. Wang, X. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 3668-77. PMID: 19265145

6. Peng, H. et al. (2011) The Journal of biological chemistry 286, 24508-18. PMID: 21586573

7. Pathak, SK. et al. (2007) Nature immunology 8, 610-8. PMID: 17486091

8. Mishra, BB. et al. (2010) Cellular microbiology 12, 1046-63. PMID: 20148899

9. Boggaram, V. et al. (2013) The Journal of biological chemistry 288, 25500-25511. PMID: 23867456

10. Sreejit, G. et al. (2014) PLoS pathogens 10, e1004446. PMID: 25356553

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mycobacterium tuberculosis esxA/ESAT-6 Antibody (TA005)

Reférences

Fiche technique

Anti-Mycobacterium tuberculosis esxA/ESAT-6 Recombinant Antibody (TA005).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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