Anti-Neisseria meningitidis NadA Polyclonal Antibody (JN971014)

Name: Anti-Neisseria meningitidis NadA Polyclonal Antibody
Brand: abinScience
SKU: JN971014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

JN971014

Description

Anti-Neisseria meningitidis NadA Polyclonal Antibody (JN971014) is a rabbit polyclonal antibody detecting autotransporter adhesin NadA in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Neisseria meningitidis

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Neisseria meningitidis NadA (Ala39-Ala170).

Cible

autotransporter adhesin NadA, partial, autotransporter adhesin NadA, partial

Purification

Purified by antigen affinity column.

Numéro d'accès

P0DV44

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Neisseria adhesin A (NadA) is a ~42 kDa protein. Adheres to and induces bacterial uptake by human epithelial cells in a microfilament-dependent process. Binding is reduced by pronase treatment, suggesting there is a protein receptor on the human cells. Possible human protein receptors include integrin beta-1 (ITGB1) and oxidized low-density lipoprotein receptor 1 (OLR1) (Probable). Binds to extracellular human Hsp90 (preferentially the beta isoform, HSP90AB1) on monocytes, binding stimulates monocytes in a TLR4-dependent fashion, polymixin B, which binds NadA, blocks the activation. Hsp90 is probably not the first receptor on human monocytes.

1. Capecchi, B. et al. (2005) Molecular microbiology 55, 687-98. PMID: 15660996

2. Liguori, A. et al. (2018) mBio 9. PMID: 30327444

3. Cecchini, P. et al. (2011) PloS one 6, e25089. PMID: 21949862

4. Bozza, G. et al. (2014) PloS one 9, e110047. PMID: 25347845

5. Comanducci, M. et al. (2002) The Journal of experimental medicine 195, 1445-54. PMID: 12045242

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Neisseria meningitidis NadA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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