Neisseria meningitidis NadA Recombinant Protein (N-His) (JN971012)

Name: Neisseria meningitidis NadA Recombinant Protein (N-His)
Brand: abinScience
SKU: JN971012
Price: 211 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.	JN971012
Description	Recombinant Neisseria meningitidis NadA Protein, N-His (JN971012) expressed in E. coli, spanning Ala39-Ala170. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P0DV44
Protein length	Ala39-Ala170
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Neisseria meningitidis
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.36 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	autotransporter adhesin NadA, Neisseria adhesin A, NadA
Background	Neisseria adhesin A (NadA) is a ~42 kDa protein. Adheres to and induces bacterial uptake by human epithelial cells in a microfilament-dependent process. Binding is reduced by pronase treatment, suggesting there is a protein receptor on the human cells. Possible human protein receptors include integrin beta-1 (ITGB1) and oxidized low-density lipoprotein receptor 1 (OLR1) (Probable). Binds to extracellular human Hsp90 (preferentially the beta isoform, HSP90AB1) on monocytes, binding stimulates monocytes in a TLR4-dependent fashion, polymixin B, which binds NadA, blocks the activation. Hsp90 is probably not the first receptor on human monocytes. 1. Capecchi, B. et al. (2005) Molecular microbiology 55, 687-98. PMID: 15660996 2. Liguori, A. et al. (2018) mBio 9. PMID: 30327444 3. Cecchini, P. et al. (2011) PloS one 6, e25089. PMID: 21949862 4. Bozza, G. et al. (2014) PloS one 9, e110047. PMID: 25347845 5. Comanducci, M. et al. (2002) The Journal of experimental medicine 195, 1445-54. PMID: 12045242
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Neisseria meningitidis NadA Protein, N-His
Bioactivity

Detected by Anti-Neisseria meningitidis NadA Antibody (F3) (Catalog No: JN971023) in indirect ELISA.